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Authentication of Human Cell Line by STR DNA Profiling Analysis

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Over the past 60 years, there has been a progressive increase in the use of cells as models, substrates, and tools for basic research and industrial applications. The rapid growth in areas like cell biology, genomics, and proteomics has triggered a remarkable increase in cell culture activities, consequently increasing the potential risk of cell line contamination, misidentification, and genetic drift. This ultimately leads research results to false interpretations, correlations, and conclusions. To avoid such risks, a simple molecular technique, Short Tandem Repeat (STR) DNA profiling for human cell line authentication test, is available. If applied routinely in cell culture management can significantly improve the detection of cellular cross-contamination resulting in more accurate assays. Discovery of HeLa Cell Line In 1951, Henrietta Lacks' cervical cancer biopsy led to the discovery of the immortal HeLa cell line. An immortal human cell line refers to a cluster of cells that ...

Raising The Bar of Cell Line Authentication Tests

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Cell lines are essential for development and research. Since the early 20th century, animal cell lines have been used for studies in medicine, cell biology, genetics, cancer research, and drug development. Later, cell lines derived from significant cell culture collections or from fellow researchers have been used to develop vaccines, drugs, reconstructive medicine, and HIV treatment and testing. A Cell Line Authentication DNA test in India is relatively straightforward: genetic analysis can be performed by using polymorphic short tandem repeats (STRs). Several companies and laboratories are developing core facilities for STR analysis. The data generated by this technique are reproducible and conform to guidelines set by leading scientific organizations. What is Cell Line Authentication DNA Test in India? Cell-line authentication DNA Test in India is the process of verifying the identity of the cells used in your experiments. Testing often includes confirming the cell lines are derive...